Fig. 3

Gel images following two-dimensional electrophoresis (2DE) in the crude extract (a) and the phosphoprotein-enriched fraction (b). No spots with clear differences in expression were observed in the gels following 2DE using the crude extract (a). However, the expression intensity of the spots enclosed by the dotted circles seemed to be greater in the 3 ppm than in the 100 ppm Fe group (b). Further, the density of the most acidic spot (arrowheads) was remarkably higher in the 3 ppm compared with the 100 ppm Fe group