Fig. 2

Expression of GHR mRNA and protein in rat DA. a Quantitative RT-PCR analyses of GHR. The expression level of GHR mRNA was maximal at E19 and E21 in the DA (n = 6–8). b Immunohistological analysis of GHR protein in the rat DA at E19, E21, and Day0. GHR was detected in a brown color. Dark blue stain was counterstained with Mayer’s hematoxylin. Scale bars 50 mm. *p < 0.05, ***p < 0.001. Data are expressed as means ± SEM. E19 Embryonic day 19, E21 embryonic day 21, Day0 at birth, DA ductus arteriosus, AO aorta, GHR growth hormone receptor, NS not significant