Fig. 4

The effect of nifedipine on the calcium transient. To apply and wash out nifedipine, embryos (n = 5) were continuously perfused with DMEM. A Representative ventral view of a rat embryo just removed from the uterus. B Fluorescence image when the embryo was observed in a small incubator. This picture corresponds to the dotted rectangle in A. C The change in rate. Open circles represent the rate calculated from the fluorescence movie, and filled circles represent the rate calculated from the visible light movie. The hatched box represents the period of nifedipine (1 μM) application. D Fluorescence recordings of the change in calcium transient corresponding to the white rectangle in B and to several times shown in C. Arrowheads indicate the peak of the calcium transient