Fig. 3

Proliferative effects of Con A and Con A plus verapamil on splenic lymphocytes from control (n = 7 per group) and alloxan-induced diabetic (n = 7 per group) mice. Lymphocytes were stimulated for 72 h with Con A or Con A plus verapamil, and proliferation was measured by MTT assay. Verapamil was added 10 min before exposure to Con A. The net ΔOD₅₇₀ is the difference between the increase in OD₅₇₀ (optical density at 570 nm) at 72 h after addition of Con A or Con A plus verapamil, and the vehicle, respectively. Data express group mean ± SEM (7 independent experiments performed in duplicate). Statistically significant difference is indicated as: **P < 0.0001 versus control group