Fig. 3

V370G mutation resulted in non-selective cation conduction in Kv1.2 channels. Cells expressing WT (a) or V370G (b) Kv1.2 channels were given a pre-pulse of +70 mV to maximally open the channels, followed by voltage steps from −120 to +20 mV at 10 mV increments to yield tail currents. c Instantaneous I–V relationships of the tail currents of WT and V370G. d Reversal potentials of the tail currents of WT and V370G. Results are mean ± SEM from 7 to 8 cells of each group. *Significantly different from WT (p < 0.05). A cell expressing V370G Kv1.2 channels was examined for tail currents in normal 140 mM Na⁺-containing bath solution (e). The same cell was exposed to a bath solution in which Na⁺ ions were completely replaced by 280 mM sucrose (f). g Instantaneous I–V relationships of the tail currents of V370G in normal and Na⁺-free bath solutions. h Reversal potentials of the tail currents of V370G in normal and Na⁺-free bath solutions. Results are mean ± SEM from 3 cells of each group. *Significantly different from the 140 Na⁺ group (p < 0.05)