Fig. 4
a Bovine PAECs were pre-treated without (control) or with 10−6 M VIP for 5–7 min. Lysates were analyzed by Western blot with antibodies to p-eNOS-Ser1177 and eNOS. The panel included the same experiment twice on the left and on the right. b Phosphorylated Ser1177 eNOS was significantly increased in the VIP-treated group compared to the control group. Bovine PAECs were treated with 10−6 M VIP for 5–7 min. Data represent mean ± SE, where control groups were normalized as one from four experiments. *P < 0.05 versus control group (0 M VIP)