Fig. 6

Western blotting showing visfatin expression in skeletal muscle tissues and skeletal muscle cell culture medium. Visfatin protein (a) and mRNA (b) expression in gastrocnemius, soleus and extensor digitorum longus (EDL) in Wistar-Kyoto rats (WKY), spontaneously hypertensive rats (SHR), and stroke-prone spontaneously hypertensive rats (SHR-SP). NS Not significant versus WKY. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was used as a loading control. c Visfatin was detected in skeletal muscle tissue and L6 rat skeletal muscle cell culture medium. Recombinant mouse visfatin was used as a positive control