Table 1 Primer sequences and amplicon lengths
Type | Gene | Direction | Primer sequence (5′ to 3′) | Product size (bp) | Accession number | |
|---|---|---|---|---|---|---|
L-type | α1S | 1 st PCR | Forward | 5′-GGCCATGCTCCCCCTGTT-3′ | 210 | AB374360a |
Reverse | 5′-CACTCGCTGCCGTTGATGG-3′ | |||||
2nd PCR | Forward | 5′-ACATCGCCCTGCTCGTCCTCT-3′ | 190 | |||
Reverse | 5′-CACTCGCTGCCGTTGATGG-3′ | |||||
α1C | 1st PCR | Forward | 5′-ACAGCGGAAGCGGCAGCAGTAT-3′ | 336 | NM_012517 | |
Reverse | 5′-AGGTAAGCGTTGGGGTGGAAGAGT-3′ | |||||
2nd PCR | Forward | 5′-AGCGGAAGCGGCAGCAGTATG-3′ | 233 | |||
Reverse | 5′-GGAGTTGGTGGCGTTGGAGTCAT-3′ | |||||
α1D | 1st PCR | Forward | 5′-ATGGTCCCCCTCCTCCACATAG-3′ | 346 | NM_017298 | |
Reverse | 5′-ATGGCCACTCCCATCCTATCG-3′ | |||||
2nd PCR | Forward | 5′-GGTCCCCCTCCTCCACATAGC-3′ | 212 | |||
Reverse | 5′-CGACCCAGCCGCTCCTACATT-3′ | |||||
α1F | 1st PCR | Forward | 5′-AAGACGGAGGACTCAGCACAACAA-3′ | 387 | NM_053701 | |
Reverse | 5′-CTGAACAGCCCGACTACAACGAT-3′ | |||||
2nd PCR | Forward | 5′-GCACAACAAGCACAAGACCGTAGT-3′ | 320 | |||
Reverse | 5′-GAATGTAGGCGCTGGGATGGA-3′ | |||||
P/Q-type | α1A | 1st PCR | Forward | 5′-GCCCAACAACGGCATCACTCA-3′ | 250 | NM_012918 |
Reverse | 5′-AAAAGCCCTTCGGTTCTCTACACG-3′ | |||||
2nd PCR | Forward | 5′-GCCCAACAACGGCATCACTCA-3′ | 193 | |||
Reverse | 5′-ACCCAGCACAAGGTTCAGCATAA-3′ | |||||
N-type | α1B | 1st PCR | Forward | 5′-TGTACAACCCCATCCCAGTCAAG-3′ | 400 | NM_147141 |
Reverse | 5′-AGGGTGCGCAGATCAAAGTCAGTT-3′ | |||||
2nd PCR | Forward | 5′-TGTACAACCCCATCCCAGTCAAG-3′ | 266 | |||
Reverse | 5′-GCCCGCCTCGAAGCAAAAG-3′ | |||||
R-type | α1E | 1st PCR | Forward | 5′-CCTCCGGGCTGTGCGTGTC-3′ | 292 | NM_019294 |
Reverse | 5′-GGGGCCGATCCAGTCCTTAC-3′ | |||||
2nd PCR | Forward | 5′-TTCTGCTCTTCTTTGCCATTCTCAT-3′ | 177 | |||
Reverse | 5′-GGCCGATCCAGTCCTTACATTCATA-3′ | |||||
T-type | α1G | 1st PCR | Forward | 5′-GCATGCGCATTCTCGTCACATTA-3′ | 394 | NM_031601 |
Reverse | 5′-TCGCCCGCAGAGCAGTTG-3′ | |||||
2nd PCR | Forward | 5′-ATGCGCATTCTCGTCACATTACT-3′ | 216 | |||
Reverse | 5′-GGGGCTCTCGTCCTCATTCTCT-3′ | |||||
α1H | 1st PCR | Forward | 5′-ATCTGCTCCTCCCGCCGTGAC-3′ | 428 | NM_153814 | |
Reverse | 5′-AGCTGGTTTTCCCTTTGCTTTGT-3′ | |||||
2nd PCR | Forward | 5′-ATCTGCTCCTCCCGCCGTGAC-3′ | 275 | |||
Reverse | 5′-ACCCAGCCCTCCAGTGTGATG-3′ | |||||
α1I | 1st PCR | Forward | 5′-CCTCTCAAAGCCATCAACCGTGTA-3′ | 367 | NM_020084 | |
Reverse | 5′-GCCCCGCCCCGAAGTCATA-3′ | |||||
2nd PCR | Forward | 5′-CTTCTTCATCTTCGGCATCATTGG-3′ | 262 | |||
Reverse | 5′-CCCGCCCCGAAGTCATACAC-3′ | |||||
GnRH | Forward | 5′-ACTGATGGCCGCTGTTGTTCT-3′ | 256 | NM_012767 | ||
Reverse | 5′-CTTCTTCTGCCCAGCTTCCTCTTCA-3′ | |||||