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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Endogenous transforming growth factor (TGF) beta1 promotes differentiation of smooth muscle cells from embryonic stem cells: stable plasmid-based siRNA silencing of TGF beta1 gene expression

Fig. 4

TGF-beta1 knockdown resulted in downregulated expression of SMC markers (SM alpha-actin and myocardin) in differentiated EBs. a RT-PCR (left panel) and quantification (right panel) of SM alpha-actin and myocardin mRNA in EBs of the TIR1 (TGF-beta1 knockdown) and LIR1 (pDS_control vector) groups with GAPDH as loading control. b Western blot (left panel) and quantification (right panel) of SM alpha-actin and myocardin expression in EBs of the TIR1 and LIR1 groups with beta-actin as loading control. *p < 0.05, **p < 0.01

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The Journal of Physiological Sciences

ISSN: 1880-6562

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