Fig. 3From: Endogenous transforming growth factor (TGF) beta1 promotes differentiation of smooth muscle cells from embryonic stem cells: stable plasmid-based siRNA silencing of TGF beta1 gene expressionRetarded extension of cell outgrowth from TGF-beta1 knockdown EBs in three-dimensional Matrigel. After differentiation for 2 days, D5 EBs cultured in Matrigel sprouted (up arrow) in EBs of the LIR1 group (pDS_control vector) whereas little sprouting could be seen in EBs of the TIR1 group (TGF-beta1 knockdown). Scale bar 100 μmBack to article page