Fig. 5

Effects of staurosporine. a The effects of PDAc on fEPSPs in the presence of 1 μM staurosporine. The numbered double lines indicate image sampling 1 and 2. Reagents were bath-applied for the indicated periods. b Summary of the effects on the first fEPSP amplitude of the treatments: vehicle alone (first left, n = 5 slices), 4α-phorbol (second left, n = 7 slices), PDAc (third left, n = 8 slices) and staurosporine plus PDAc (right most, n = 12 slices). *P < 0.01 and **P < 0.0005 (Mann–Whitney U-test). c Summary of the effects of PDAc on the paired-pulse ratio of fEPSPs in the absence (left, n = 8 slices) and the presence of staurosporine (right, n = 12 slices). Open column, paired-pulse ratio before PDAc treatment; closed column, 10 minutes after exposure to PDAc. *P < 0.01 (Wilcoxon signed-ranks test). d Average fluorescence profiles of LMFBs (n = 65 boutons, 15 slices) in the presence of staurosporine before (top), after PDAc (middle) and the difference (bottom). e Cumulative probability plots of the ratio-2/1 value of ΔSpH; vehicle alone (black line), PDAc (blue line) and staurosporine plus PDAc (magenta line)