Fig. 4

Effects of BPE on the rate of SpH recycling. a The ΔSpH signals were normalized and averaged before (closed circles) and after 5 μM bafilomycin A1 (open diamonds) (mean ± SEM, n = 30 boutons, 7 slices). b The contribution of SpH recycling through the processes of endocytosis-reacidification was estimated by the subtraction of the ΔSpH before bafilomycin A1 from that after, and was plotted (gray squares, mean ± SEM, n = 30 boutons, 7 slices). c Each ΔSpH signal before (top left) and after PDAc (top right) was normalized to the value at the end of a train of repetitive stimulations (bottom traces) and a regression line (blue) was fitted to the data of 0–5 s after the last stimulation. d Summary (mean ± SEM) of the SpH recycling rate before (open columns) and after vehicle alone (DMSO, left filled column, n = 27 boutons, 4 slices) or PDAc (right filled column, n = 44 boutons, 10 slices)