Fig. 5

Effects of pharmacologic inhibition of nNOS by L-VNIO and genetic deletion of nNOS on IP-induced reduction of cardiac apoptosis. a Representative TUNEL-positive staining for each group as described in “Methods”. Green spots indicate positive apoptosis nuclei whereas blue indicates individual nuclei. b Percentage of positive apoptosis nuclei and total nuclei expressed as mean ± SEM. c Caspase-3 activities were measured as described in “Methods”, and are expressed as mean ± SEM. White bars represent WT mice groups; black bars represent KO groups. ¶P < 0.05 compared with the WT IR group, †P < 0.05 compared with the WT IP group