Fig. 2

Effects of L-VNIO and MnTBAP on IR-induced cardiac apoptosis. a Representative TUNEL-positive staining for each group, as described in “Methods”. Green spots indicate positive apoptosis nuclei and blue indicates individual nuclei. b Percentage of positive apoptosis nuclei and total nuclei expressed as mean ± SEM. c Caspase-3 activities were measured as described in “Methods”, and are expressed as mean ± SEM. White bars represent WT mice groups; black bars represent KO groups. *P < 0.05 compared with the wild Sham group, ¶P < 0.05 compared with the WT IR group, †P < 0.05 compared with the WT IR + LVNIO group, §P < 0.05 compared with the KO IR group