Fig. 9
Expression of nNOS protein in NE-MD cells pre-treated under various conditions (Western blotting). a Representative staining of nNOS protein expression (from left to right) in Na+ Ringer (lane 1), furosemide-treatment (lane 2), furosemide-treatment plus EIPA (lane 3), furosemide-treatment plus DMSO (lane 4), 1/4 Cl− (lane 5), 1/10 Cl− (lane 6), 1/4 Na+ (lane 7), and 1/10 Na+ (lane 8). Various levels of a ~65-kDa band were observed. Staining of corresponding GAPDH was used for normalization; 2 μg protein in each lane. b The relative staining values are 0.52 ± 0.15 (Na+ Ringer), 1 [+Furo (control)], 0.59 ± 0.14 (+Furo+EIPA), 0.93 ± 0.21 (+Furo+DMSO), 0.75 ± 0.22 (1/4 Cl−), 1.16 ± 0.21 (1/10 Cl−), 0.51 ± 0.08 (1/4 Na+), and 0.57 ± 0.04 (1/10 Na+). **P < 0.02 compared with control. *P < 0.05 compared with Na+ Ringer (1st column). The experimental number is 4 except for lanes 3 and 4 of n = 3