Fig. 3

Down-regulating effect of various hormones on the expression of WNK4 gene. COS-7 cells were treated with the above hormones [lane 1 estrogen (final concentration 1 μmol/l), 2 insulin (100 nmol/l), 3 dexamethasone (1 nmol/l), 4 angiotensin II (5 μmol/l), 5 growth hormone (6 nmol/l), 6 blank control] for 24 h. RNA of the cells were then extracted with the Trizol method and quantified with RT-PCR