Table 2 Primers used for RT-PCR and multi-cell RT-PCR
From: Gene structures, biochemical characterization and distribution of rat melatonin receptors
Gene | Direction | Primer sequences | Product size (bp) |
|---|---|---|---|
RT-PCR | |||
MT1 | Forward | 5′-ATGGCCCTGGCTGTGCTGCGGTAAG-3′ | 316 |
Reverse | 5′-TAAGTATAGACGTCAGCGCCAAGGGAAATG-3′ | ||
MT2 | Forward | 5′-GGAGCGCCCCCAAGCAGTG-3′ | 390 |
Reverse | 5′-GGATCTCCCCAAGTACCCAACCGTCAT-3′ | ||
β-Actin | Forward | 5′-GTCCACACCCGCCACCAGT-3′ | 496 |
Reverse | 5′-CGTCTCCGGAGTCCATCACAAT-3′ | ||
mGAPDH | Forward | 5′-TGAAGGTCGGTGTGAACGGATTTG-3′ | 359 |
Reverse | 5′-GGCGGAGATGATGACCCTTTTG-3′ | ||
Multi-cell RT-PCR | |||
MT1 | |||
1st PCR | Forward | 5′-ATGGCCCTGGCTGTGCTGCGGTAAG-3′ | 442 |
Reverse | 5′-TGTGGCAAATGTAGCAGTAGCGGTTCA-3′ | ||
2nd PCR | Forward | 5′-CAGGCGGCGGGGAGGAAATAAG-3′ | 231 |
Reverse | 5′-TAAGTATAGACGTCAGCGCCAAGGGAAATG-3′ | ||
MT2 | |||
1st PCR | Forward | 5′-GGAGCGCCCCCAAGCAGTG-3′ | 390 |
Reverse | 5′-GGATCTCCCCAAGTACCCAACCGTCAT-3′ | ||
2nd PCR | Forward | 5′-CGGGCTGCAGCGTCACCAT-3′ | 275 |
Reverse | 5′-GTCAGCCAAGGCCAGATTCACC-3′ | ||
GnRH | Forward | 5′-ACTGATGGCCGCTGTTGTTCT-3′ | 256 |
Reverse | 5′-CTTCTTCTGCCCAGCTTCCTCTTCA-3′ | ||