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Table 2 Primers used for RT-PCR and multi-cell RT-PCR

From: Gene structures, biochemical characterization and distribution of rat melatonin receptors

Gene Direction Primer sequences Product size (bp)
RT-PCR  
 MT1 Forward 5′-ATGGCCCTGGCTGTGCTGCGGTAAG-3′ 316
Reverse 5′-TAAGTATAGACGTCAGCGCCAAGGGAAATG-3′
 MT2 Forward 5′-GGAGCGCCCCCAAGCAGTG-3′ 390
Reverse 5′-GGATCTCCCCAAGTACCCAACCGTCAT-3′
 β-Actin Forward 5′-GTCCACACCCGCCACCAGT-3′ 496
Reverse 5′-CGTCTCCGGAGTCCATCACAAT-3′
 mGAPDH Forward 5′-TGAAGGTCGGTGTGAACGGATTTG-3′ 359
Reverse 5′-GGCGGAGATGATGACCCTTTTG-3′
Multi-cell RT-PCR  
 MT1  
  1st PCR Forward 5′-ATGGCCCTGGCTGTGCTGCGGTAAG-3′ 442
Reverse 5′-TGTGGCAAATGTAGCAGTAGCGGTTCA-3′
  2nd PCR Forward 5′-CAGGCGGCGGGGAGGAAATAAG-3′ 231
Reverse 5′-TAAGTATAGACGTCAGCGCCAAGGGAAATG-3′
 MT2
  1st PCR Forward 5′-GGAGCGCCCCCAAGCAGTG-3′ 390
Reverse 5′-GGATCTCCCCAAGTACCCAACCGTCAT-3′
  2nd PCR Forward 5′-CGGGCTGCAGCGTCACCAT-3′ 275
Reverse 5′-GTCAGCCAAGGCCAGATTCACC-3′
 GnRH Forward 5′-ACTGATGGCCGCTGTTGTTCT-3′ 256
Reverse 5′-CTTCTTCTGCCCAGCTTCCTCTTCA-3′
  1. Forward and reverse primers were designed on different exons to distinguish the amplification of cDNA templates from that of contaminating genomic DNA. To design the PCR primers, we referred to the following nucleotide sequences: AB377274 for MT1, AB377275 for MT2, NM_031144 for β-actin, NM_008084 for mGAPDH, and M12579 for GnRH