Fig. 7

Treatment with a calcineurin inhibitor abolishes the inhibition of brown adipocyte differentiation by Yoda-1. A Representative images of Oil Red O staining in differentiated brown adipocytes following application of 10 μM Yoda-1 with or without 5 μM FK506. Vehicle indicates differentiation medium with solvent (0.2% DMSO). Scale bar: 100 μm. B The triglyceride levels following application of 10 μM Yoda-1 with or without 5 μM FK506 in differentiated brown adipocytes. Each data was normalized to that of vehicle (absorbance; 0.180 ± 0.024). Each column represents the mean + SEM of 5–7 experiments. Statistical significance was assessed using ANOVA followed by two-tailed multiple t-tests with Bonferroni correction. *p < 0.05 vs. vehicle, ^##p < 0.01 vs. Yoda-1. C The calcineurin activities following application of 10 μM Yoda-1 with or without 20 μM Dooku-1 in inducted brown adipocytes. Each column represents the mean + SEM of 6–7 experiments. Statistical significance was assessed using ANOVA followed by two-tailed multiple t-tests with Bonferroni correction. *p < 0.05 vs. vehicle, ^##p < 0.01 vs. Yoda-1