Fig. 6

Knockdown of Piezo1 prevents the suppression of brown adipocyte differentiation by Yoda-1. A Representative images of Oil Red O staining in differentiated brown adipocytes transfected with scrRNA (upper) or Piezo1 siRNA (lower). The cells were treated with (right) or without (Vehicle, left) 10 μM Yoda-1. Scale bar: 100 μm. B The triglyceride levels in differentiated brown adipocytes transfected with scrRNA or Piezo1 siRNA, and treated with or without 10 μM Yoda-1. The triglyceride levels were normalized to that in the scrRNA-vehicle (absorbance; 0.219 ± 0.005). Each column represents the mean + SEM of 6 experiments. Statistical significance was assessed by two-way ANOVA (no significant interaction, p = 0.159), followed by Student’s t-test. scrRNA vs. Piezo1 siRNA, p < 0.001; Vehicle vs. 10 μM Yoda-1, p = 0.0018. C The inhibition ratio calculated by dividing the absorbance of Yoda-1 by the absorbance of vehicle using the data in B. Each column represents the mean + SEM of 6 experiments. Statistical significance was assessed using Student’s t-test. **p < 0.01 vs. scrRNA