Fig. 2

The functional expression of Piezo1 in brown adipocytes. A–D Representative Ca²⁺-imaging traces of changes in the intracellular Ca²⁺ concentration ([Ca²⁺]_i) induced by 10 μM Yoda-1, a Piezo1 agonist, in pre-adipocytes (Day 0, A), inducted brown adipocytes (Day 2, B), and differentiated brown adipocytes on days 4 (C) and 8 (D). Ionomycin (5 μM, Iono) was used to confirm cell viability. E Representative traces of changes in [Ca²⁺]_i induced by 10 μM Yoda-1 with 20 μM Dooku-1, a Piezo1 antagonist, in pre-adipocytes (Day 0). F Summary of [Ca²⁺]_i changes 90 s after application of 10 μM Yoda-1 with or without 20 μM Dooku-1 in pre-adipocytes (Day 0). Each column represents the mean + SEM of 102–118 cells. Statistical significance was assessed using Student’s t-test. **p < 0.01 vs. Yoda-1