Skip to main content
Fig. 5 | The Journal of Physiological Sciences

Fig. 5

From: Early donepezil monotherapy or combination with metoprolol significantly prevents subsequent chronic heart failure in rats with reperfused myocardial infarction

Fig. 5

Immunohistological staining of macrophages on day 8 post-RMI. Representative micrographs of CD68 (a), and triple CD68, vWF, and DAPI staining of the I/R region (b). Representative macrographs of CD68 and CD80 cells (proinflammatory M1) (c), and CD68 and CD163 cells (anti-inflammatory M2) (d) in the I/R region. Scale bars, 50 μm. e Quantitative analysis of macrophage infiltration (UT:24 ± 3%/field, n = 4, 24 fields of view; DT: 11 ± 2%/field, n = 6, 36 fields; MT: 24 ± 2%/field, n = 5, 30 fields; DMT: 10 ± 3%/field, n = 4, 32 fields). f M1 infiltration (UT: 9.4 ± 0.8%/field, n = 4, 24 fields; DT: 1.6 ± 0.9%/field, n = 5, 30 fields; MT: 9.8 ± 0.5%/field, n = 5, 30 fields; DMT: 1.5 ± 0.2%/field, n = 4, 24 fields). g M2 infiltration (UT: 1.5 ± 0.1%/field, n = 4, 24 fields; DT: 3.1 ± 0.5%/field, n = 5, 30 fields; MT: 1.5 ± 0.2%/field, n = 5, 30 fields; DMT: 4.8 ± 0.6%/field, n = 4, 24 fields). h M1 to M2 ratio (UT: 6.9 ± 1.7, n = 4; DT: 1.1 ± 0.6, n = 5; MT: 6.0 ± 0.9, n = 5; DMT: 0.4 ± 0.1, n = 4). M1, macrophage type 1; M2, macrophage type 2. Data are expressed as means ± SEMs. e–h Box-and-whisker plots based on macrophage, M1, and M2 infiltration and the M1/M2 ratio in all fields. *P < 0.05, **P < 0.01 vs. UT; †P < 0.05, ††P < 0.01 vs. MT, using nonparametric Kruskal–Wallis tests, followed by Dunn’s test. I/R, ischemia/reperfusion

Back to article page