Fig. 2

“Spent time” on the thermal gradient ring. A “Spent time” of all genotypes at each temperature zone from 11.5 ℃ to 43.6 ℃ over a 60-min period (wild type n = 15, TRPA1^−/− n = 14, TRPM2^−/− n = 13, TRPAM8^−/− n = 14, TRPV1^−/− n = 11, TRPV3^−/− n = 13, TRPV4^−/− n = 15). The gray box shows an enlarged view of the lower temperatures. *, **p < 0.05, p < 0.01 vs. wild type. B Transition of peak “Spent time” temperature in each genotype in the first, middle, and last 20 min of the experiment. The color of each genotype is the same as shown in panel A. Mice were classified into two groups, as shown by the brackets. C “Spent time” in each temperature zone of wild-type (green), TRPM2^−/− (orange), and TRPA1^−/− (purple) mice under the condition of 11.5 ℃ to 43.6 ℃ during the last 20 min (40–60 min). p values are shown on the table. D “Spent time” in each temperature zone of wild-type (green) and TRPA1^−/− (purple) mice under the condition of 19.7 ℃ to 35.3 ℃ during the last 20 min. (wild type n = 12, TRPA1^−/− n = 14, TRPM2^−/− n = 12, TRPAM8^−/− n = 12, TRPV1^−/− n = 11, TRPV3^−/− n = 13, TRPV4^−/− n = 15). All error bars represent standard errors of the mean. Colors of asterisks and ns (not significant) indicate the comparison between wild-type mice and mice lacking TRP channels, as shown in panel A. *p < 0.05, **p < 0.01 vs. wild type