Fig. 5
Defective autophagy in VSMCs increased S100A4, vinculin, but not paxillin. Immunohistochemical staining (a) and western blot analysis (b) of S100A4 in aortic segments of Atg7+/+ SM22α-Cre+ (+/+) or Atg7F/F SM22α-Cre+ (F/F) animals (n = 6). Amount of vinculin (red, c) and paxillin (d) determined after immunohistochemical staining of aorta segments and Atg7+/+ SM22α-Cre+ (+/+) or Atg7F/F SM22α-Cre+ (F/F) mice (n = 6). Two-way ANOVA on 2 segments per mice *p < 0.05 for genotype factor. Scale bar = 100 µm