Table 1 Primer pairs used to amplify ion transporters and channels
From: Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles
Name | Accession number | Primer | Sequence (5′ → 3′) | Size (bp) |
|---|---|---|---|---|
Cftr | NM_021050.2 | Forward | (4070) atggaaagttgcagatgaggtt | 399 |
Reverse | (4468) ctcatcttttccgaggagctaa | |||
Slc26a3 (Dra) | NM_021353.3 | Forward | (556) cctactttttcttgggcacatc | 385 |
Reverse | (940) accgactccaggactttgaata | |||
Slc26a4 (Pendrin) | NM_011867.4 | Forward | (974) cggcatcctctccattatctac | 465 |
Reverse | (1438) gccacaaaacaggagaaaaatc | |||
Slc26a6 (Pat1) | NM_134420.4 | Forward | (2455) tgaaagagaagtgcggtgtaga | 385 |
Reverse | (2839) ttcttcaggctcttaatgcaca | |||
Slc26a9 | NM_177243.4 | Forward | (1457) cactgacccctactacctctgg | 425 |
Reverse | (1881) tggttttcatgaagagggactt | |||
α-ENaC | NM_011324.2 | Forward | (1496) caggcgaattattctcagttcc | 451 |
Reverse | (1946) ccttgggcttagggtagaagat | |||
β-ENaC | NM_001272023.1 | Forward | (1004) acatcggtcaggaggactatgt | 282 |
Reverse | (1285) ggtcttggaaacaggaatgaag | |||
γ-ENaC | NM_011326.3 | Forward | (709) gaagaaactggtgggatttcag | 367 |
Reverse | (1075) gaaggggttgtactcatcttcg | |||
Slc4a2 (Ae2) | NM_001253892.1 | Forward | (3298) aacccaagattcaggaagtcaa | 471 |
Reverse | (3768) tctcgttgtactcatccacacc | |||
Slc4a3 (Ae3) | NM_001357149.1 | Forward | (1612) atgaccctgatgctaaggagaa | 393 |
Reverse | (2004) gaatcacaatgctaccatccaa | |||
Slc4a4 (NBCe1) | NM_001136260.1 | Forward | (2206) aaaaccagtcgctattttccaa | 412 |
Reverse | (2617) gggcaatggagataacagtagc | |||
Slc4a5 (NBCe2) | NM_001166067.1 | Forward | (1814) agcctcttatcatcctcagcag | 315 |
Reverse | (2128) tgtaggtggtgatgaagtcagg | |||
Slc4a7 (NBCn1) | NM_001033270.2 | Forward | (676) accctatgtggcaactctgtct | 393 |
Reverse | (1068) ttttctctgcttcctccacttc | |||
Slc4a8 (NDCBE) | NM_001347102.1 | Forward | (381) gtttgaagaggatgtggaggac | 434 |
Reverse | (814) gatccaccttgcttagatccac | |||
Slc4a10 (NBCn2) | NM_001242378.1 | Forward | (2967) gtgcttcgtctctcaaaggaat | 445 |
Reverse | (3412) cacatggcagtctttgacattt | |||
Slc9a1 (Nhe1) | NM_134647.4 | Forward | (951) gtgcctgatagcaggagagc | 202 |
Reverse | (1153) ccttgtccttggacagtgct | |||
Slc9a2 (Nhe2) | NM_001033289.2 | Forward | (2088) gcacagtcttcgggaaagtc | 168 |
Reverse | (2256) gtccgagtcgctgctatttc | |||
Slc9a3 (Nhe3) | NM_001081060.2 | Forward | (2046) acagaagcggaggaatagca | 199 |
Reverse | (2245) tatcaattcctgccccagag | |||
Slc9a4 (Nhe4) | NM_177084.3 | Forward | (2084) gaggaacctgccaaaatcaa | 162 |
Reverse | (2246) ccacgtcttcaggagaaagc | |||
Slc9a5 (Nhe5) | NM_001323971.2 | Forward | (970) ggacaggtgggaacagtttg | 182 |
Reverse | (1152) ggcatagagggcagagtgag |