Fig. 8
From: Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles
Apical HCO3− efflux in bronchiole epithelial cells from CF mice. a–c Isolated bronchioles from ΔF/ΔF mice were first bilaterally perfused with the standard HCO3−-buffered solution and the luminal perfusate was switched to the standard Hepes-buffered HCO3−–CO2-free solution. After HCO3−–CO2 was removed from the luminal perfusate, forskolin (5 μM, b) or ACh (10 μM, c) was applied to the lumen. Means ± SD of 5–6 experiments, respectively. Blue lines indicate mean changes of pHi in wild-type bronchioles as references. d Basal pHi in the presence of HCO3−–CO2 in wild-type (n = 8) and ΔF/ΔF (n = 6) bronchioles. #p < 0.05. e Transient increase of pHi (ΔpH) by removal of luminal HCO3−–CO2 in wild-type and ΔF/ΔF bronchioles. #p < 0.01. f Early-phase pHi decline (ΔpH for 1 min) just after stimulation with forskolin or acetylcholine. #p < 0.01 compared to wild-type. *p < 0.01, **p < 0.05 compared with control (without stimulation). &p < 0.01. g Late-phase pHi decline (ΔpH/min at midpoint pHi of 6.95) under stimulation with forskolin or ACh (red dashed lines in a–c). *p < 0.05 compared with control (without stimulation). #p < 0.05 compared to wild-type