Fig. 4
From: Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles
Effects of luminal Cl− removal on pHi in bronchiole epithelial cells. a–d Isolated bronchioles were first bilaterally perfused with the standard Hepes-buffered HCO3−–CO2-free solution (a) or the standard HCO3−-buffered solution (b–d). Luminal Cl− was removed by replacement with gluconate in the absence (a, b) or presence of luminal forskolin (5 μM) (c) or luminal H2DIDS (200 μM) (d). Time course changes of pHi are shown as means ± SD of 8 experiments, respectively. e The activity of apical Cl−–HCO3− exchange is shown as the initial rate of pHi increase upon luminal Cl− removal (red dashed lines in a–d). *p < 0.01