Fig. 3
From: Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles
Effects of luminal application of forskolin, CFTRinh-172, H2DIDS, and amiloride on apical HCO3− efflux. a–e Isolated bronchioles were first bilaterally perfused with the standard HCO3−-buffered solution and the luminal perfusate was switched to the standard Hepes-buffered HCO3−–CO2-free solution. After HCO3−–CO2 was removed from the luminal perfusate, forskolin (5 μM) was applied to the lumen. Time course changes of pHi in the absence (a) or presence of CFTRinh-172 (5 μM) in the lumen (b), H2DIDS (200 μM) in the lumen (c), both CFTRinh-172 (5 μM) and H2DIDS (200 μM) in the lumen (d), or amiloride (1 μM) in the lumen (e) are shown as means ± SD of 8 experiments, respectively. The blue line in a indicates mean change of pHi without forskolin stimulation as a reference. f Early-phase pHi decline (ΔpH for 1 min) just after forskolin stimulation. #p < 0.01 compared with control (without forskolin stimulation). *p < 0.01 compared with forskolin alone. &p < 0.05. g Late-phase pHi decline (ΔpH/min at midpoint pHi of 6.95) under forskolin stimulation (red dashed lines in a-e). #p < 0.05 compared with control (without forskolin stimulation). *p < 0.01, **p < 0.05 compared with forskolin alone. &p < 0.01