Fig. 3

PDP1 upregulation reserves the inhibitory effect of miR-18a-3p overexpression on levels of inflammatory cytokines and matrix metalloproteinases in IL-1β-stimulated ATDC5 cells. A, B PDP1 protein level in IL-1β-stimulated ATDC5 cells after overexpressing miR-18a-3p or PDP1 was examined by western blotting. C–E The secretion of inflammatory cytokines IL-8, IL-6 and PGE2 in IL-1β-stimulated ATDC5 cells after overexpressing miR-18a-3p or PDP1 was examined by ELISA. F Protein levels of matrix metalloproteinases (MMP2, MMP3 and MMP9) in IL-1β-stimulated ATDC5 cells after overexpressing miR-18a-3p or PDP1 were examined by western blotting. **p  < 0.01, ***p  < 0.001