Fig. 2

MiR-200c-3p overexpression promoted EndMT in ox-LDL-treated HUVECs. HUVECs were treated with ox-LDL at different concentrations (0, 10, 20, 40 μg/mL) for 48 h. A The expression of miR-200c-3p in HUVECs was assessed by qRT-PCR. B HUVECs were transfected with miR-200c-3p mimic or mimic NC. The expression of miR-200c-3p in the HUVECs was assessed by qRT-PCR. HUVECs were transfected with miR-200c-3p mimic or mimic NC, followed by 40 μg/mL ox-LDL treatment. C Rhodamine phalloidin staining was performed to observe cell morphology of HUVECs. The blue arrows indicate cobblestone-like cells, and the red arrows indicate the spindle-like cells. D WB was performed to estimate the expression of CD31, vWF, α-SMA, and vimentin in the HUVECs. **P < 0.01 vs. mimic NC or ox-LDL + mimic NC group; ^##P < 0.01 vs. 0 or Control group