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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Chemogenetic activation of endogenous arginine vasopressin exerts anorexigenic effects via central nesfatin-1/NucB2 pathway

Fig. 4

Suppressed food intake induced by chemogenetic activation of AVP neurons was attenuated after i.c.v. administered nesfatin-1/NucB2-neutralizing antibody (-NA) without any alteration of water intake. A Digital image of an implanted stainless-steel cannulae aimed at the lateral ventricle. Vehicle (8 µg/4 µL) or Nesfatin-1/NucB2-neutralizing antibody (-NA) (8 µg/4 µL) was i.c.v. administered at 30 min prior to the start of the dark cycle, at ZT 11.5. Just after the intracerebroventricular injection, Saline or CNO (1 mg/mL/kg) was subcutaneously administered. The measurements were commenced at ZT 0. Cumulative food (g) (B) and water intake (mL) (C), and their intake per unit time (g/h, mL/h) (D, E) were calculated from the results. All rats had free access to food and water during the measurements. Data are presented as means ± SEM (Saline s.c. + Vehicle i.c.v., n = 7; Saline s.c. + nesfatin-1/NucB2-NA i.c.v., n = 8; CNO s.c. + Vehicle i.c.v., n = 8; CNO s.c. + nesfatin-1/NucB2-NA i.c.v., n = 10). *P < 0.05, **P < 0.01 vs. Saline s.c. + Vehicle i.c.v. #P < 0.05, ##P < 0.01 vs. CNO s.c. + Vehicle i.c.v

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