Fig. 3

Effect of PKC activation on hLAT2 mRNA and protein expression levels. a The effect of PKC activation on hLAT2 mRNA expression levels in S2-Mock and S2-LAT2 cells was examined by qPCR. Total RNA was isolated from the cells that had been treated with DMSO (0.1% (v/v), -) or PMA (0.1 or 1 µM) for 30 min. The values were normalized to the mGapdh mRNA expression level, and the data are shown as the mean ± SD of values obtained from three separate experiments, each performed in duplicate. NS, not significant. b The effect of PKC activation on hLAT2 protein expression levels in S2-Mock and S2-LAT2 cells was examined by Western blot analysis. Whole cell lysates were prepared from cells that had been treated in the same way as explained in a. β-Actin was used as a loading control, and representative results that were obtained from three independent experiments are shown. c The effect of PKC activation on hLAT2 mRNA expression levels in Caco-2 cells was examined by qPCR. Total RNA was isolated from the cells that had been treated with DMSO (0.1% (v/v)) or PMA (1 µM) for 4 h. The values were normalized to the hGAPDH mRNA expression level, and the data are shown as the mean ± SD of values obtained from three separate experiments, each performed in duplicate. NS, not significant. d The effect of PKC activation on hLAT2 protein expression levels in Caco-2 cells was examined by Western blot analysis. Whole cell lysates were prepared from cells that had been treated in the same way as explained in c. β-Actin was used as a loading control, and representative results that were obtained from three independent experiments are shown