Fig. 3
Immunohistochemistry for c-Fos protein in the brain. Representative results of immunohistochemistry for c-Fos protein in the hypothermic rat (a, b), active control rat (c, d) and anesthetized control rat (e, f) are shown. a, c, e a low-magnification view of the brain. Scale bar = 2 mm. b, d, f a high-magnification view of the hypothalamus. Arrows show immunoreactivity for c-Fos protein. In the hypothermic rat, c-Fos-positive cells were confined to the VMPO of the hypothalamus. Scale bar = 500 µm. 3 V, 3rd ventricle; MnPO, median preoptic nucleus, VMPO, ventromedial preoptic nucleus; MPA, medial preoptic area. g shows the numbers of c-Fos-positive cells in the VMPO of the hypothermic rat, active control rat and anesthetized control rat. Values are means ± SD (n = 3). The blue horizontal lines denote statistical significance (p < 0.05)