Fig. 3

TP73 was a target of miR-34a-5p. a Prediction of target relationship between miR-34a-5p and CASC7 or TP73. Luciferase reporter assay was performed to evaluate the interaction ability between miR-34a-5p and CASC7 or TP73 3′-UTR. b, d TP73 mRNA expression determined by qRT-PCR analysis, and c, e TP73 protein level determined by western blot in human PTC cell lines (K1 and TPC-1) co-transfected with miR-34a-5p mimic/mimic NC and miR-34a-5p inhibitor/inhibitor NC. *P < 0.05, **P < 0.01 vs. mimic NC; ^&&P < 0.01 vs. inhibitor NC. Data are expressed as the mean ± standard deviation (n = 3).