Fig. 4
Spontaneous transient inward currents (STICs) recorded in human primary uterine cultured cells are ANO1-mediated. a Representative tracings of spontaneous transient inward currents (STICs) recorded at different holding potentials (Vm). Inset are STICs displayed at a higher resolution time scale. b Compiled I/V relationship (mean peak amplitude of STICs vs. voltage holding potential) demonstrates the observed reverse potential for STICs is − 7.5 ± 10 mV (n = 4), which closely approximates the predicted reversal potential for chloride (ECl of − 13.8 mV). c Representative whole-cell membrane STICs recordings from human USM cells perfused with either vehicle buffer (upper tracing) or with a TMEM16A (ANO1) antibody for use on live cells (lower tracing). Abrogation of STIC activity was observed (14 of 20 cells patched) following incubation with antibody compared to incubation with vehicle (3 of 20 patched cells). d Comparison of STIC amplitude between vehicle-treated or TMEM16A (ANO1) antibody-treated cells. There was significant attenuation of STIC amplitude by 82 ± 6% compared with vehicle controls (**p < 0.01)