Table 2 Antibodies
Antigen | Host | Label | Dilution | Source |
|---|---|---|---|---|
Primary antibody | ||||
EP2 receptor | Rabbit | 1:100 | Acris Antibodies | |
EP4 receptor | Rabbit | 1:100 | Cayman | |
α-smooth muscle actin | Mouse | 1:500 | Sigma-Aldrich | |
HU | ||||
GFAP | Mouse | 1:2000 | Chemicon | |
Secondary antibody | ||||
Rabbit IgG | Donkey | Alexa Fluor 594 | 1:200 | Molecular Probes |
Mouse IgG | Goat | Alexa Fluor 488 | 1:200 | Molecular Probes |
Goat IgG | Donkey | Alexa Fluor 488 | 1:200 | Molecular Probes |