Fig. 3
Three-dimensional imaging of pial artery and Aβ obtained before, during and after common carotid artery occlusion. An example image obtained from a wild-type (WT) mouse is illustrated with a maximal intensity projection (a). To determine the artery diameter, the pial artery was segmented with a volume of interest (VOI) of 10-μm width (a). As an example, three consecutive VOI images from two separated locations before, during, and after carotid artery occlusion are presented (b, c). An example image of pial artery obtained from an APPNL-G-F mouse is presented with a maximum intensity projection (d). VOIs on Aβ-present site (orange) and Aβ-absent site (white) are identified. As an example, three consecutive VOI images from Aβ-present site (e) and Aβ-absent site (f) before, during, and after carotid artery occlusion are presented. Note that slice images are presented in e to clarify the diameter change of artery covered with Aβ. Scale bar indicates 50 μm