Fig. 4From: Segmental differences in Slc26a3-dependent Cl− absorption and HCO3− secretion in the mouse large intestine in vitro in Ussing chambersThe alkaline secretion rates (J(OH)) induced by Cl− and by propionate in the mucosal solution. J(OH) values were measured by titrating mucosal solution with a pH stat device in the isolated cecum (a) or middle-distal colon (b) mounted in Ussing chambers. The serosal side was always bathed with HCO3−-containing buffered solutions, the same solution as used for flux measurements. Left-hand figures; slc26a3(+/+ or +/−) and right-hand figures; slc26a3(−/−). J(OH) was first measured under Cl−-free and then 70 mM Cl−-containing mucosal solution to determine the Cl−-induced HCO3− secretion rate. In some tissues, J(OH) was additionally measured under 70 mM Cl− plus 65 mM propionate-containing mucosal solution to determine the propionate-dependent HCO3− secretion rate. The same symbols between the cecum and mid-distal colon indicate that tissues were obtained from the same animals. Horizontal bars connected with a dotted line are the mean values. In the middle-distal colon, but not in the cecum, the J(OH) under Cl−-free conditions was significantly different between the two groups (unpaired t test, p < 0.01). The results are presented as µEq cm−2 h−1. [Additional information; in the left figures the filled circles are slc26a3(+/−), while other symbols are slc26a3(+/+).]Back to article page