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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: Segmental differences in Slc26a3-dependent Cl absorption and HCO3 secretion in the mouse large intestine in vitro in Ussing chambers

Fig. 3

Unidirectional fluxes of 22Na from mucosal to serosal solution (Jms) and serosal to mucosal solution (Jsm) were determined using two adjacent tissues under short-circuit conditions, Jnet being Jms minus Jsm values. The fluxes in the cecum (a), proximal colon (0–30% length of the entire colon) (b), and middle-distal colon (c) were compared between the slc26a3(+/+ or +/−) (unfilled square) and slc26a3(−/−) (filled square) mice. These experiments were conducted in the presence of benzamil (10 μM, mucosal side) to suppress electrogenic Na+ absorption. The results are presented as µEq cm−2 h−1. Data are the mean ± SE. Statistical comparisons were performed by unpaired t test. *0.01 < p < 0.05 between slc26a3(+/+ or +/−) and slc26a3(−/−). n = 4 for the slc26a3(+/+ or +/−) mice and n = 4–5 for the slc26a3(−/−) mice. Additional information; the numbers of the slc26(+/−) [vs. slc26a3(+/+)] in the control group are 1 (vs. 3) in the cecum, (0 vs. 5) in the proximal colon and 0 (vs. 4) in the middle-distal colon. Jms, Jsm, and Jnet from the slc26(+/−) were 14.8, 2.5 and 12.3 in the cecum (in µEq cm−2 h−1)

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