Fig. 3From: Segmental differences in Slc26a3-dependent Cl− absorption and HCO3− secretion in the mouse large intestine in vitro in Ussing chambersUnidirectional fluxes of 22Na from mucosal to serosal solution (Jms) and serosal to mucosal solution (Jsm) were determined using two adjacent tissues under short-circuit conditions, Jnet being Jms minus Jsm values. The fluxes in the cecum (a), proximal colon (0–30% length of the entire colon) (b), and middle-distal colon (c) were compared between the slc26a3(+/+ or +/−) (unfilled square) and slc26a3(−/−) (filled square) mice. These experiments were conducted in the presence of benzamil (10 μM, mucosal side) to suppress electrogenic Na+ absorption. The results are presented as µEq cm−2 h−1. Data are the mean ± SE. Statistical comparisons were performed by unpaired t test. *0.01 < p < 0.05 between slc26a3(+/+ or +/−) and slc26a3(−/−). n = 4 for the slc26a3(+/+ or +/−) mice and n = 4–5 for the slc26a3(−/−) mice. Additional information; the numbers of the slc26(+/−) [vs. slc26a3(+/+)] in the control group are 1 (vs. 3) in the cecum, (0 vs. 5) in the proximal colon and 0 (vs. 4) in the middle-distal colon. Jms, Jsm, and Jnet from the slc26(+/−) were 14.8, 2.5 and 12.3 in the cecum (in µEq cm−2 h−1)Back to article page