Fig. 5

Phagocytosis of fluorescent beads by the WT and Msn-KO microglia following treatment with 10 μM UDP. a Representative images of the WT and Msn-KO microglia are shown. Fluorescent beads are shown in green, and nuclei labeled with propidium iodide (PI) are depicted in red. Scale bar, 40 μm. High magnification images obtained by confocal microscopy are presented in the insets, in which fluorescent beads, Iba1 and PI were shown in green, blue and red, respectively. b The number of fluorescent beads per WT and Msn-KO microglia was counted in the presence and absence of 10 μM UDP, and shown by closed (without UDP) and open columns (with UDP), respectively. 150–200 cells were studied in each experiment. Results are shown as mean ± SEM. *P < 0.05 vs. WT (n = 3 trials)