Fig. 4

Membrane ruffling in microglia. The WT (a, b, c) and Msn-KO microglia (d, e, f) were treated with serum-free DMEM (as a control) (a, d), 50 μM ADP (b, e) or UDP (c, f) for 5 min at 37℃. After fixation, the cells were permeabilized with PBS containing 0.1% Triton X-100, and stained with rhodamine phalloidin to observe ruffle membranes (shown by arrow heads)