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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: Moesin is involved in microglial activation accompanying morphological changes and reorganization of the actin cytoskeleton

Fig. 3

a Confocal microscopic images of primary microglia stained with an anti-Iba1 antibody. a The WT (a, b, c) and Msn-KO (g, h, i) microglia were treated with serum-free DMEM (as a control) (a, g), 50 μM ADP (b, h) or UDP (c, i) for 5 min at 37℃, and stained with an anti-Iba1 antibody. b Their phase contrast images were also shown (d, e, f, and j, k, l). b The area stained with anti-Iba1 antibody was calculated with ImageJ software. Closed, open, and hatched columns represent serum-free DMEM, ADP and UDP treatment, respectively. Results are shown as mean ± SEM. * p < 0.01 vs. WT treated with serum-free DMEM (n = 3), ** p < 0.05 vs. WT treated with serum-free DMEM (n = 3), and # p < 0.05 vs. WT treated with serum-free DMEM (n = 3)

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