Fig. 2

Expression of ERM proteins in primary microglia. a The expression of Iba1 (a and e, in green) and moesin (b and f, in red) and their merged pattern (c and g) in the WT (a, b, c) and Msn-KO microglia (e, f, g) was studied by immunofluorescence. Their phase contrast images were also shown (d, h). Scale bars, 40 μm. b The cell lysates of WT and Msn-KO microglia were blotted with an antibody that recognizes all three ERM proteins and with an antibody that detects β-actin (bottom). (Left) The WT-microglia abundantly expressed moesin with a molecular mass of 75 kDa, whereas they expressed very small amounts of ezrin (*) and radixin (**). (Right) The Msn-KO microglia lose moesin without apparent compensatory upregulation of ezrin and radixin