Fig. 7
Effects of FFA on the TTX-insensitive component of action potential firing in unstimulated AVP neurons. a–c Representative records of spontaneous action potential firing before and after application of 0.5 μM tetrodotoxin (TTX) and then of 100 μM FFA (a), 3 mM Ni2+ (b), or 0.5 μM ω-CgTx (c) in the presence of TTX in AVP neurons exposed to normotonic solution containing 2 mM Ca2+. Inset panels given below the traces of membrane potential changes represent expanded traces for the indicated parts of the records. d The averages of firing frequency (Hz) in the absence (Control) and presence of TTX (+ TTX) or TTX plus FFA (+ FFA), TTX plus ω-CgTx (+ ω-CgTx), or TTX plus Ni2+ (+ Ni2+). *P < 0.05 vs. Control (n = 7–24). #P < 0.05 vs. TTX alone