Fig. 6
Effects of flufenamic acid (FFA) on VGCC currents in unstimulated AVP neurons. a Representative record of currents before and after application of 100 μM FFA (upper panel) in AVP neurons exposed to normotonic solution containing 50 mM Ba2+. Inset panels given below the current trace represent expanded current responses to step pulses from – 60 to + 70 mV applied at i and ii. b, c Representative records of currents before and after application of FFA in the absence and presence of 0.5 μM ω-CgTx (b) or 3 mM Ni2+ (c). Inset panels given below the current traces represent expanded traces of current responses to step pulses from – 60 to + 70 mV applied at i, ii and iii. d Percent currents at − 10 mV (left panel) and + 20 mV (right panel) in the presence of FFA (n = 9), ω-CgTx (n = 11), ω-CgTx plus FFA (n = 11), Ni2+ (n = 8), and Ni2+ plus FFA (n = 8) compared to the control currents (Control) in the absence of these blockers. *P < 0.05 vs. Control. #P < 0.05 vs. ω-CgTx alone or Ni2+ alone