Fig. 4
Effects of Ni2+ and ML218 on VGCC currents in unstimulated AVP neurons. a Representative record of currents before and after application of a low concentration (10 μM) or a high concentration (3 mM) of Ni2+ to AVP neurons exposed to normotonic solution containing 50 mM Ba2+. Inset panels given below the current trace represent expanded current responses to step pulses from − 60 to + 70 mV applied at i, ii and iii. b Current–voltage relationships for peak VGCC currents (n = 8–12). *P < 0.05 vs. Control. c Percent currents at − 10 mV (left panel) and + 20 mV (right panel) after perfusion of control solutions (n = 8) containing no blockers (drug-free: n = 8), 10 μM Ni2+ (n = 12), 3 mM Ni2+ (n = 8), and 10 μM ML218 (n = 9) compared to the control currents (Control) recorded before perfusion. *P < 0.05 vs. Control. #P < 0.05 vs. drug-free