Fig. 5
ATP concentration and GLUT1 expression in HUVECs. a Intracellular ATP production was measured following culture of the cells under normoxia or hypoxia, with or without glibenclamide (Glb, 10 μM), as described in the Materials and Methods. Data are expressed as the mean ± SEM from 4 independent experiments. *P < 0.01 vs. normoxic control without glibenclamide treatment (Mann–Whitney test). b HUVECs in 96-well plates were treated with antimycin-A (Anti-A, 10 μM) or 2-deoxyglucose (2-DG, 1 mM) for 30 min at normoxia. In some groups, the cells were also treated with glibenclamide (Glb, 10 μM). ATP was measured as described in the Materials and Methods. Data are from experiments performed in duplicate. *P < 0.01 compared with control without glibenclamide treatment (Mann–Whitney test). c HUVECs were treated with CoCl2 (100 μM) with or without glibenclamide (Glb, 10 μM). Intracellular ATP concentration was analyzed as described in the “Materials and methods”. Data are expressed as the mean ± SEM from 4 independent experiments. No statistical significance was detected by the Mann–Whitney test. d GLUT1 expression determined by immunoblotting following treatment with antimycin-A (Anti-A, 10 μM), 2-deoxyglucose (2-DG, 1 mM), CoCl2 (100 µM), and/or glibenclamide (Glb, 10 μM). Data are expressed as the mean ± SEM from 4 independent experiments. #P < 0.05 versus vehicle control without glibenclamide treatment (Mann–Whitney test)