Fig. 1

Glucose uptake and GLUT1 expression in HUVECs under normoxia and hypoxia. a Glucose uptake of HUVECs under normoxia and hypoxia. HUVECs were exposed to hypoxia, and glucose uptake was measured as described in the Materials and methods. Data are expressed as the mean ± SEM from 6 independent experiments. *P < 0.05 (Mann–Whitney test). b GLUT1 and GLUT3 expression in HUVECs under normoxia and hypoxia determined by immunoblotting. Data are expressed as the mean ± SEM of 12 samples from 3 independent experiments. *P < 0.01 vs. normoxia control of each condition (Mann–Whitney test). c Effect of the GLUT1 inhibitor BAY876 on glucose uptake in HUVECs. HUVECs were cultured under normoxia or hypoxia with BAY876 as described in the Materials and Methods. Data are expressed as a percentage of glucose uptake compared with vehicle control in each condition. Data are expressed as the mean ± SEM from 5 independent results. *P < 0.01 (one-way ANOVA). d Effect of GLUT1 inhibitor on sprouting of cells from choroid explants [16]. The explants were treated with vehicle or the GLUT1 inhibitor BAY876 for 4 days. Images were taken and the area of cell sprouting was quantified by ImageJ software. Data are the mean ± SEM from 12 explants in 2 time-independent experiments with similar results. *P < 0.01 (one-way ANOVA with Dunnett’s correction). e GLUT1 expression in HUVECs under normoxia and hypoxia determined by immunoblotting. Data are expressed as the mean ± SEM from 6 independent experiments. Glb, glibenclamide (10 μM). *P < 0.01 vs. normoxia control of each condition (Mann–Whitney test)