Fig. 1

TRPM7 silencing attenuates the increment in [Mg²⁺]_i of H9c2 cells by incubation in high-Mg²⁺ solution. A Each symbol represents mean ± SE of [Mg²⁺]_i obtained from shControl cells or shTRPM7 cells. Cells were perfused with Ca²⁺-free Tyrode’s solution (1 mM Mg²⁺), except during the period of perfusion with high-Mg²⁺ (95 mM) solution indicated by the line. B Total number of the cells (a) and transfection efficiency (b) are plotted as a function of time after transfection with non-targeting (black columns) or TRPM7 (white columns) shRNA. Each column shows mean ± SE of the data from 3 different experiments. There was no statistical difference of cell number (shControl vs shTRPM7) in any time between 24 and 144 h. C Quantitative RT-PCR analysis of TRPM7 mRNA expression relative to that of GAPDH. Each column shows mean ± SE of the data obtained from, respectively, shControl cells (1.0 ± 0.051, n = 4) or shTRPM7 cells (0.407 ± 0.026, n = 4). D Western blot analysis of protein expression of TRPM7 in shControl and shTRPM7 cells. E Relative fluorescence intensities of furaptra from shControl or shTRPM7 cells are plotted against time after administration of Ni²⁺. *0.01 ≤ p < 0.05, **p < 0.01