Fig. 7

Ca²⁺ uptake through reverse mode of NCXm in isolated mitochondria. a, b Ca²⁺ uptake in the depolarized mitochondria. Intra-mitochondrial Ca²⁺ was measured in Na⁺-unloaded mitochondria (0 Na⁺), Na⁺-loaded mitochondria (Na⁺-loaded) and Na⁺-loaded mitochondria with 20 μM CGP-37157 (Na⁺-loaded + CGP). a Representative data. b Initial rates of intra-mitochondrial Ca²⁺ rise (n = 4). *p < 0.05. c, d Ca²⁺ uptake in energized mitochondria. Intra-mitochondrial Ca²⁺ uptake was measured in control (control), in the presence of Ru360 (+ Ru360) and in the presence of ruthenium red (+ RuR). c Representative data. d Initial rates of intra-mitochondrial Ca²⁺ rise (n = 4). *p < 0.05