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Fig. 9 | The Journal of Physiological Sciences

Fig. 9

From: Class II phosphatidylinositol 3-kinase α and β isoforms are required for vascular smooth muscle Rho activation, contraction and blood pressure regulation in mice

Fig. 9

FRET imaging of attenuated Rho activation in C2α- and C2β-depleted HASM. a Effects of C2α and C2β knockdown on the expression of IMC (0.3µM)-induced Rho activation as evaluated with FRET imaging in control and C2α- and C2β-double depleted HASM. Left, representative images. Right, quantified data of time-dependent Rho-FRET signals. b Effects of C2α and C2β knockdown on ET-1 (1 µM)-induced Rho activation as evaluated with FRET imaging in control and C2α- and C2β-double depleted HASM. Left, representative images. Right, quantified data of time-dependent Rho-FRET signals. c Partial co-localization of RhoA-FRET signals with the early endosome marker Rab5. Cells were transfected with BFP-Rab5 expression vector and the co-localization of RhoA-FRET signals and BFP-Rab5 was determined

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